The DNA damage sensor ATM kinase interacts with the p53 mRNA and guides the DNA damage response pathway.

ABSTRACT:

Background

The ATM kinase constitutes a master regulatory hub of DNA damage and activates the p53 response pathway by phosphorylating the MDM2 protein, which develops an affinity for the p53 mRNA secondary structure. Disruption of this interaction prevents the activation of the nascent p53. The link of the MDM2 protein-p53 mRNA interaction with the upstream DNA damage sensor ATM kinase and the role of the p53 mRNA in the DNA damage sensing mechanism, are still highly anticipated.

Methods

The proximity ligation assay (PLA) has been extensively used to reveal the sub-cellular localisation of the protein-mRNA and protein-protein interactions. ELISA and co-immunoprecipitation confirmed the interactions in vitro and in cells.

Results

This study provides a novel mechanism w

SUBMITTER: Karakostis K 

PROVIDER: S-EPMC10804554 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature