PICLS with human cells is the first high throughput screening method for identifying novel compounds that extend lifespan.

ABSTRACT: Gerontology research on anti-aging interventions with drugs could be an answer to age-related diseases, aiming at closing the gap between lifespan and healthspan. Here, we present two methods for assaying chronological lifespan in human cells: (1) a version of the classical outgrowth assay with quantitative assessment of surviving cells and (2) a version of the PICLS method (propidium iodide fluorescent-based measurement of cell death). Both methods are fast, simple to conduct, cost-effective, produce quantitative data for further analysis and can be used with diverse human cell lines. Whereas the first method is ideal for validation and testing the post-intervention reproductive potential of surviving cells, the second method has true high-throughput screening potential. The new technolog

SUBMITTER: Alfatah M 

PROVIDER: S-EPMC10804585 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature