Comprehensive analysis of m6A modification in lipopolysaccharide-induced acute lung injury in mice.

ABSTRACT:

Background

N6-Methyladenosine (m6A) methylation is the most prevalent post-transcriptional modification in mRNA, and plays significant roles in various diseases. Nevertheless, the precise functions of m6A modification in the formation of ALI remain unclear. In this study we explore the transcriptome distribution of m6A methylation and its probable roles of in ALI.

Methods

Lipopolysaccharide (LPS) was utilized to establish an ALI mouse model. Real-time qPCR, Western blotting and m6A dot blot were utilized to assess m6A methylation level and the expression of m6A methylation enzymes. MeRIP-Seq and RNA-seq were utilized to explore differential m6A modifications and differentially expressed genes in ALI mice. The hub genes and enriched pathways were assessed by Real-time qPCR an

SUBMITTER: Xu C 

PROVIDER: S-EPMC10804706 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature