An Expanded Genetic Toolbox to Accelerate the Creation of <i>Acholeplasma laidlawii</i> Driven by Synthetic Genomes.

ABSTRACT: We have developed genetic tools for the atypical bacterium Acholeplasma laidlawii. A. laidlawii is a member of the class Mollicutes, which lacks cell walls, has small genomes, and has limited metabolic capabilities, requiring many metabolites from their hosts. Several of these traits have facilitated the development of genome transplantation for some Mollicutes, consequently enabling the generation of synthetic cells. Here, we propose the development of genome transplantation for A. laidlawii. We first investigated a donor-recipient relationship between two strains, PG-8A and PG-8195, through whole-genome sequencing. We then created multihost shuttle plasmids and used them to optimize an electroporation protocol. We also evolved a superior strain for DNA

SUBMITTER: Nucifora DP 

PROVIDER: S-EPMC10805103 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature