Modularized Design and Construction of Tunable Microbial Consortia with Flexible Topologies.

ABSTRACT: Complex and fluid bacterial community compositions are critical to diversity, stability, and function. However, quantitative and mechanistic descriptions of the dynamics of such compositions are still lacking. Here, we develop a modularized design framework that allows for bottom-up construction and the study of synthetic bacterial consortia with different topologies. We showcase the microbial consortia design and building process by constructing amensalism and competition consortia using only genetic circuit modules to engineer different strains to form the community. Functions of modules and hosting strains are validated and quantified to calibrate dynamic parameters, which are then directly fed into a full mechanistic model to accurately predict consortia composition dynamics for both a

SUBMITTER: Chen X 

PROVIDER: S-EPMC10805104 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature