Toolbox of Characterized Genetic Parts for <i>Staphylococcus aureus</i>.

ABSTRACT: Staphylococcus aureus is an important clinical bacterium prevalent in human-associated microbiomes and the cause of many diseases. However, S. aureus has been intractable to synthetic biology approaches due to limited characterized genetic parts for this nonmodel Gram-positive bacterium. Moreover, genetic manipulation of S. aureus has relied on cumbersome and inefficient cloning strategies. Here, we report the first standardized genetic parts toolbox for S. aureus, which includes characterized promoters, ribosome binding sites, terminators, and plasmid replicons from a variety of bacteria for precise control of gene expression. We established a standard relative expression unit (REU) for S. aureus using a plasmid reference and characterized genetic parts

SUBMITTER: Rondthaler SN 

PROVIDER: S-EPMC10805105 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature