Synthetic Homoserine Lactone Sensors for Gram-Positive <i>Bacillus subtilis</i> Using LuxR-Type Regulators.

ABSTRACT: A universal biochemical signal for bacterial cell-cell communication could facilitate programming dynamic responses in diverse bacterial consortia. However, the classical quorum sensing paradigm is that Gram-negative and Gram-positive bacteria generally communicate via homoserine lactones (HSLs) or oligopeptide molecular signals, respectively, to elicit population responses. Here, we create synthetic HSL sensors for Gram-positive Bacillus subtilis 168 using allosteric LuxR-type regulators (RpaR, LuxR, RhlR, and CinR) and synthetic promoters. Promoters were combinatorially designed from different sequence elements (-35, -16, -10, and transcriptional start regions). We quantified the effects of these combinatorial promoters on sensor activity and determined how regulator expression af

SUBMITTER: Zeng M 

PROVIDER: S-EPMC10805106 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature