A kinetic model for solving a combination optimization problem in ab-initio Cryo-EM 3D reconstruction.

ABSTRACT: Cryo-Electron Microscopy (cryo-EM) is a widely used and effective method for determining the three-dimensional (3D) structure of biological molecules. For ab-initio Cryo-EM 3D reconstruction using single particle analysis (SPA), estimating the projection direction of the projection image is a crucial step. However, the existing SPA methods based on common lines are sensitive to noise. The error in common line detection will lead to a poor estimation of the projection directions and thus may greatly affect the final reconstruction results. To improve the reconstruction results, multiple candidate common lines are estimated for each pair of projection images. The key problem then becomes a combination optimization problem of selecting consistent common lines from multiple candidates. To solv

SUBMITTER: Liu J 

PROVIDER: S-EPMC10805181 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature