Modulation of canonical Wnt signaling regulates peribiliary mesenchymal identity during homeostasis and injury.

ABSTRACT:

Background

The matrix and associated mesenchyme of the extrahepatic bile ducts are distinct, which could drive diseases with a predilection for these ducts, such as primary sclerosing cholangitis. We aimed to understand the molecular drivers of peribiliary mesenchymal cell (PMC) identity in the extrahepatic bile ducts and dissect how this changed in the context of injury using an entirely in vivo approach with transcriptomic analysis.

Methods and results

Single-cell sequencing with a receptor-ligand analysis showed that PMCs had the most interactions with surrounding cells. Wnt4, Wnt5a, and Wnt7b were identified as the major ligands secreted from PMCs and cholangiocytes that interacted in both paracrine and autocrine fashion. Bile duct ligation caused an increase in all 3 Wi

SUBMITTER: Singh S 

PROVIDER: S-EPMC10805418 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature