Matrixed CDR grafting: A neoclassical framework for antibody humanization and developability.

ABSTRACT: Discovery and optimization of a biotherapeutic monoclonal antibody requires a careful balance of target engagement and physicochemical developability properties. To take full advantage of the sequence diversity provided by different antibody discovery platforms, a rapid and reliable process for humanization of antibodies from nonhuman sources is required. Canonically, maximizing homology of the human variable region (V-region) to the original germline was believed to result in preservation of binding, often without much consideration for inherent molecular properties. We expand on this approach by grafting the complementary determining regions (CDRs) of a mouse anti-LAG3 antibody into an extensive matrix of human variable heavy chain (VH) and variable light chain (VL) framework regions wit

SUBMITTER: Gupta P 

PROVIDER: S-EPMC10805677 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature