TRPP2 is located in the primary cilia of human non-pigmented ciliary epithelial cells.

ABSTRACT:

Purpose

Mechanosensitive channels (MSCs) and primary cilium possess a possible relevance for the sensation of intraocular pressure (IOP). However, there is only limited data on their expression and localization in the ciliary body epithelium (CBE). The purpose of this study was to characterize the expression and localization of TRPP2 in a human non-pigmented ciliary epithelial cell (HNPCE) line.

Methods

The expression of the TRPP2 was studied by quantitative (q)RT-PCR and in situ hybridization in rat and human tissue. Protein expression and distribution were studied by western blot analysis, immunohistochemistry, and immunoelectron microscopy. Cellular location of TRPP2 was determined in rat and human CBE by immunofluorescence and immunoblot analysis. Electron microscopy stu

SUBMITTER: Zheng W 

PROVIDER: S-EPMC10806040 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature