METTL1 mediated tRNA m⁷G modification promotes leukaemogenesis of AML via tRNA regulated translational control.

ABSTRACT:

Background

RNA modifications have been proven to play fundamental roles in regulating cellular biology process. Recently, maladjusted N7-methylguanosine (m⁷G) modification and its modifiers METTL1/WDR4 have been confirmed an oncogene role in multiple cancers. However, the functions and molecular mechanisms of METTL1/WDR4 in acute myeloid leukemia (AML) remain to be determined.

Methods

METTL1/WDR4 expression levels were quantified using qRT-PCR, western blot analysis on AML clinical samples, and bioinformatics analysis on publicly available AML datasets. CCK-8 assays and cell count assays were performed to determine cell proliferation. Flow cytometry assays were conducted to assess cell cycle and apoptosis rates. Multiple techniques were used for mechanism studies

SUBMITTER: Zhao P 

PROVIDER: S-EPMC10807064 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature