Co-expression of IL-21-Enhanced NKG2D CAR-NK cell therapy for lung cancer.

ABSTRACT:

Background

Adoptive cell therapy has achieved great success in treating hematological malignancies. However, the production of chimeric antigen receptor T (CAR-T) cell therapy still faces various difficulties. Natural killer (NK)-92 is a continuously expandable cell line and provides a promising alternative for patient's own immune cells.

Methods

We established CAR-NK cells by co-expressing natural killer group 2 member D (NKG2D) and IL-21, and evaluated the efficacy of NKG2D-IL-21 CAR-NK cells in treating lung cancer in vitro and in vivo.

Results

Our data suggested that the expression of IL-21 effectively increased the cytotoxicity of NKG2D CAR-NK cells against lung cancer cells in a dose-dependent manner and suppressed tumor growth in vitro and in vivo. In addition,

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC10807083 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature