Detecting prokaryote-specific gene and other bacterial signatures in thrombi from patients with acute ischemic stroke.

ABSTRACT:

Background and purpose

Microbial infection has been associated with thrombogenesis. This study aimed to detect bacterium-specific genes and other signatures in thrombi from patients with acute ischemic stroke and to relate these signatures to clinical characteristics.

Methods

Blood samples were collected before thrombectomy procedures, and thrombus samples were obtained during the procedure. Identification and classification of bacteria in the samples were accomplished using 16 S rRNA gene sequencing. Bacterium-specific structures were observed with transmission electron microscopy. Bacterium-specific biomarkers were detected through immunohistochemical staining.

Results

16 S rRNA gene was detected in 32.1% of the thrombus samples from 81 patients. Bacillus (0.04% vs.

SUBMITTER: Wang X 

PROVIDER: S-EPMC10807108 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature