Interface Engineering of Carrier-Protein-Dependent Metabolic Pathways.

ABSTRACT: Carrier-protein-dependent metabolic pathways biosynthesize fatty acids, polyketides, and non-ribosomal peptides, producing metabolites with important pharmaceutical, environmental, and industrial properties. Recent findings demonstrate that these pathways rely on selective communication mechanisms involving protein-protein interactions (PPIs) that guide enzyme reactivity and timing. While rational design of these PPIs could enable pathway design and modification, this goal remains a challenge due to the complex nature of protein interfaces. Computational methods offer an encouraging avenue, though many score functions fail to predict experimental observables, leading to low success rates. Here, we improve upon the Rosetta score function, leveraging experimental data through iterative round

SUBMITTER: Sztain T 

PROVIDER: S-EPMC10807135 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature