Single-cell landscape of the cellular microenvironment in three different colonic polyp subtypes in children.

ABSTRACT:

Background

The understanding of the heterogeneous cellular microenvironment of colonic polyps in paediatric patients with solitary juvenile polyps (SJPs), polyposis syndrome (PJS) and Peutz-Jeghers syndrome (PJS) remains limited.

Methods

We conducted single-cell RNA sequencing and multiplexed immunohistochemistry (mIHC) analyses on both normal colonic tissue and different types of colonic polyps obtained from paediatric patients.

Results

We identified both shared and disease-specific cell subsets and expression patterns that played important roles in shaping the unique cellular microenvironments observed in each polyp subtype. As such, increased myeloid, endothelial and epithelial cells were the most prominent features of SJP, JPS and PJS polyps, respectively. Noticea

SUBMITTER: Deng Y 

PROVIDER: S-EPMC10807352 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature