Engineering Novosphingobium aromaticivorans to produce cis,cis-muconic acid from biomass aromatics.

ABSTRACT: The platform chemical cis,cis-muconic acid (ccMA) provides facile access to a number of monomers used in the synthesis of commercial plastics. It is also a metabolic intermediate in the β-ketoadipic acid pathway of many bacteria and, therefore, a current target for microbial production from abundant renewable resources via metabolic engineering. This study investigates Novosphingobium aromaticivorans DSM12444 as a chassis for the production of ccMA from biomass aromatics. The N. aromaticivorans genome predicts that it encodes a previously uncharacterized protocatechuic acid (PCA) decarboxylase and a catechol 1,2-dioxygenase, which would be necessary for the conversion of aromatic metabolic intermediates to ccMA. This study confirmed the activity of

SUBMITTER: Vilbert AC 

PROVIDER: S-EPMC10807440 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature