Genetic tools for the electrotroph Sporomusa ovata and autotrophic biosynthesis.

ABSTRACT: Sporomusa ovata is a Gram-negative acetogen of the Sporomusaceae family with a unique physiology. This anerobic bacterium is a core microbial catalyst for advanced CO₂-based biotechnologies including gas fermentation, microbial electrosynthesis, and hybrid photosystem. Until now, no genetic tools exist for S. ovata, which is a critical obstacle to its optimization as an autotrophic chassis and the acquisition of knowledge about its metabolic capacities. Here, we developed an electroporation protocol for S. ovata. With this procedure, it became possible to introduce replicative plasmids such as pJIR751 and its derivatives into the acetogen. This system was then employed to demonstrate the feasibility of heterologous expression by introducing a functional β-gl

SUBMITTER: Tremblay P-L 

PROVIDER: S-EPMC10807461 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature