Rapid, high-contrast, and steady volumetric imaging with Bessel-beam-based two-photon fluorescence microscopy.

ABSTRACT:

Significance

Two-photon fluorescence microscopy (TPFM) excited by Gaussian beams requires axial tomographic scanning for three-dimensional (3D) volumetric imaging, which is a time-consuming process, and the slow imaging speed hinders its application for in vivo brain imaging. The Bessel focus, characterized by an extended depth of focus and constant resolution, facilitates the projection of a 3D volume onto a two-dimensional image, which significantly enhances the speed of volumetric imaging.

Aim

We aimed to demonstrate the ability of a TPFM with a sidelobe-free Bessel beam to provide a promising tool for research in live biological specimens.

Approach

Comparative in vivo imaging was conducted in live mouse brains and transgenic zebrafish to evaluate the

SUBMITTER: Chen Y 

PROVIDER: S-EPMC10807873 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature