Molecular determinants of the crosstalk between endosomal microautophagy and chaperone-mediated autophagy.

ABSTRACT: Chaperone-mediated autophagy (CMA) and endosomal microautophagy (eMI) are pathways for selective degradation of cytosolic proteins in lysosomes and late endosomes, respectively. These autophagic processes share as a first step the recognition of the same five-amino-acid motif in substrate proteins by the Hsc70 chaperone, raising the possibility of coordinated activity of both pathways. In this work, we show the existence of a compensatory relationship between CMA and eMI and identify a role for the chaperone protein Bag6 in triage and internalization of eMI substrates into late endosomes. Association and dynamics of Bag6 at the late endosome membrane change during starvation, a stressor that, contrary to other autophagic pathways, causes a decline in eMI activity. Collectively, these resul

SUBMITTER: Krause GJ 

PROVIDER: S-EPMC10807933 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature