MscS inactivation and recovery are slow voltage-dependent processes sensitive to interactions with lipids.

ABSTRACT: Mechanosensitive channel MscS, the major bacterial osmolyte release valve, shows a characteristic adaptive behavior. With a sharp onset of activating tension the channel population readily opens, but under prolonged action of moderate tension it inactivates. The inactivated state is non-conductive and tension insensitive, which suggests that the gate becomes uncoupled from the lipid-facing domains. Because the distinct opening and inactivation transitions are both driven from the closed state by tension transmitted through the lipid bilayer, here we explore how mutations of two conserved positively charged lipid anchors, R46 and R74, affect 1) the rates of opening and inactivation and 2) the voltage dependences of these transitions. Previously estimated kinetic rates for opening-closing tr

SUBMITTER: Britt M 

PROVIDER: S-EPMC10808034 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature