Electric field-enhanced backscatter interferometry detection for capillary electrophoresis.

ABSTRACT: Backscatter interferometry (BSI) is a refractive index (RI) detection method that is easily integrated with capillary electrophoresis (CE) and is capable of detecting species ranging from inorganic ions to proteins without additional labels or contrast agents. The BSI signal changes linearly with the square of the separation voltage which has been used to quantify sample injection, but has not been explored as a potential signal enhancement mechanism in CE. Here we develop a mathematical model that predicts a signal enhancement at high field strengths, where the BSI signal is dominated by the voltage dependent mechanism. This is confirmed in both simulation and experiment, which show that the analyte peak area grows linearly with separation voltage at high field strengths. This effect can

SUBMITTER: De Silva M 

PROVIDER: S-EPMC10808210 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature