Elevation of inositol pyrophosphate IP₇ in the mammalian spinal cord of amyotrophic lateral sclerosis.

ABSTRACT:

Background

Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disorder associated with progressive impairment of spinal motor neurons. Continuous research endeavor is underway to fully understand the molecular mechanisms associating with this disorder. Although several studies have implied the involvement of inositol pyrophosphate IP₇ in ALS, there is no direct experimental evidence proving this notion. In this study, we analyzed inositol pyrophosphate IP₇ and its precursor IP₆ in the mouse and human ALS biological samples to directly assess whether IP₇ level and/or its metabolism are altered in ALS disease state.

Methods

We used a liquid chromatography-mass spectrometry (LC-MS) protocol originally-designed for mammal

SUBMITTER: Ito M 

PROVIDER: S-EPMC10808411 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature