Open-Source System for Real-Time Functional Assessment of In Vitro Filtration Barriers.

ABSTRACT: The integrity of the barrier between blood and the selective filtrate of solutes is important for homeostasis and its disruption contributes to many diseases. Microphysiological systems that incorporate synthetic or natural membranes with human cells can mimic biological filtration barriers, such as the glomerular filtration barrier in the kidney, and they can readily be used to study cellular filtration processes as well as drug effects and interactions. We present an affordable, open-source platform for the real-time monitoring of functional filtration status in engineered microphysiological systems. Using readily available components, our assay can linearly detect real-time concentrations of two target molecules, FITC-labeled inulin and Texas Red-labeled human-serum albumin, within clin

SUBMITTER: Fallon TK 

PROVIDER: S-EPMC10808466 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature