Characterization of Klebsiella pneumoniae carrying the bla_NDM-1 gene in IncX3 plasmids and the rare In1765 in an IncFIB-IncHI1B plasmid.

ABSTRACT:

Background

Today, the bla_NDM gene is widely distributed on several plasmids from a variety of Gram-negative bacteria, primarily in transposons and gene cassettes within their multidrug-resistant (MDR) regions. This has led to the global dissemination of the bla_NDM gene.

Methods

The determination of class A beta-lactamase, class B and D carbapenemases was performed according to the recommendations of the Clinical and Laboratory Standards Institute (CLSI). Antimicrobial susceptibility testing was performed using both the BioMerieux VITEK2 system and antibiotic paper diffusion methods. Plasmid transfer was then evaluated by conjugation experiments and plasmid electroporation assays. To comprehensively analyze the complete genome of K. pneu

SUBMITTER: Ma L 

PROVIDER: S-EPMC10808583 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature