Generation of human TMEM16F-specific affibodies using purified TMEM16F.

ABSTRACT: Introduction: TMEM16 family proteins are involved in a variety of functions, including ion transport, phospholipid scrambling, and the regulation of membrane proteins. Among them, TMEM16F has dual functions as a phospholipid scramblase and a nonselective ion channel. TMEM16F is widely expressed and functions in platelet activation during blood clotting, bone formation, and T cell activation. Despite the functional importance of TMEM16F, the modulators of TMEM16F function have not been sufficiently studied. Method: In this study, we generated TMEM16F-specific affibodies by performing phage display with brain-specific TMEM16F (hTMEM16F) variant 1 purified from GnTi^- cells expressing this variant in the presence of digitonin as a detergent. Purified human TMEM16F prot

SUBMITTER: Kim E 

PROVIDER: S-EPMC10808743 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature