Single-cell transcriptomic analysis of human pleura reveals stromal heterogeneity and informs <i>in vitro</i> models of mesothelioma.

ABSTRACT: The pleural lining of the thorax regulates local immunity, inflammation and repair. A variety of conditions, both benign and malignant, including pleural mesothelioma, can affect this tissue. A lack of knowledge concerning the mesothelial and stromal cells comprising the pleura has hampered the development of targeted therapies. Here, we present the first comprehensive single-cell transcriptomic atlas of the human parietal pleura and demonstrate its utility in elucidating pleural biology. We confirm the presence of known universal fibroblasts and describe novel, potentially pleural-specific, fibroblast subtypes. We also present transcriptomic characterisation of multiple in vitro models of benign and malignant mesothelial cells, and characterise these through comparison with in v

SUBMITTER: Obacz J 

PROVIDER: S-EPMC10809128 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature