Vision-Dependent and -Independent Molecular Maturation of Mouse Retinal Ganglion Cells.

ABSTRACT: The development and connectivity of retinal ganglion cells (RGCs), the retina's sole output neurons, are patterned by activity-independent transcriptional programs and activity-dependent remodeling. To inventory the molecular correlates of these influences, we applied high-throughput single-cell RNA sequencing (scRNA-seq) to mouse RGCs at six embryonic and postnatal ages. We identified temporally regulated modules of genes that correlate with, and likely regulate, multiple phases of RGC development, ranging from differentiation and axon guidance to synaptic recognition and refinement. Some of these genes are expressed broadly while others, including key transcription factors and recognition molecules, are selectively expressed by one or a few of the 45 transcriptomically distinct types def

SUBMITTER: Whitney IE 

PROVIDER: S-EPMC10809145 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature