Identification and experimental validation of ferroptosis-related gene lactotransferrin in age-related hearing loss.

ABSTRACT:

Objective

To reveal the relationship between ARHL and ferroptosis and screen ferroptosis-related genes (FRGs) in ARHL.

Methods

Bioinformatics were used to analyze the hub genes and molecular mechanism of ferroptosis in the aging cochleae. Senescence β-galactosidase staining, iron content detection, and micro malondialdehyde (MDA) assay kits were used to measure β-galactosidase activity, and expression of Fe²⁺ and MDA, respectively. Fluorescence microscope was used for immunofluorescence assay of hub genes. Western blot was used to verify the expression of hub genes in HEI-OC1 cells, cochlear explants, and cochleae of C57BL/6J mice. Data were expressed as mean ± SD of at least three independent experiments.

Results

The analysis of bioinformatics confirmed th

SUBMITTER: Zeng C 

PROVIDER: S-EPMC10809180 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature