Development of a rapid and highly accurate method for ¹³C tracer-based metabolomics and its application on a hydrogenotrophic methanogen.

ABSTRACT: Microfluidic capillary electrophoresis-mass spectrometry (CE-MS) is a rapid and highly accurate method to determine isotopomer patterns in isotopically labeled compounds. Here, we developed a novel method for tracer-based metabolomics using CE-MS for underivatized proteinogenic amino acids. The method consisting of a ZipChip CE system and a high-resolution Orbitrap Fusion Tribrid mass spectrometer allows us to obtain highly accurate data from 1 μl of 100 nmol/l amino acids comparable to a mere 1 [Formula: see text] 10⁴-10⁵ prokaryotic cells. To validate the capability of the CE-MS method, we analyzed 16 protein-derived amino acids from a methanogenic archaeon Methanothermobacter thermautotrophicus as a model organism, and the mass spectra showed sharp peaks wit

SUBMITTER: Fukuyama Y 

PROVIDER: S-EPMC10809761 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature