A NanoCurvS platform for quantitative and multiplex analysis of curvature-sensing proteins.

ABSTRACT: The cell membrane is characterized by a rich variety of topographical features such as local protrusions or invaginations. Curvature-sensing proteins, including the Bin/Amphiphysin/Rvs (BAR) or epsin N-terminal homology (ENTH) family proteins, sense the bending sharpness and the positive/negative sign of these topographical features to induce subsequent intracellular signaling. A number of assays have been developed to study curvature-sensing properties of proteins in vitro, but it is still challenging to probe low curvature regime with the diameter of curvature from hundreds of nanometers to micrometers. It is particularly difficult to generate negative membrane curvatures with well-defined curvature values in the low curvature regime. In this work, we develop a nanostructure-based

SUBMITTER: Lu CH 

PROVIDER: S-EPMC10809791 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature