Real-Time Imaging of Single Retinal Cell Apoptosis in a Non-Human Primate Ocular Hypertension Model.

ABSTRACT:

Purpose

To evaluate the feasibility of using DARC (detection of apoptosing retinal cells) technology as a biomarker for preclinical assessment of glaucomatous damage in a non-human primate (NHP) model of ocular hypertension (OHT).

Methods

Elevated intraocular pressure (IOP) was induced by applying a laser to the trabecular meshwork in each eye of NHPs. Changes in DARC counts in the retina, identified as fluorescent-tagged annexin V (ANX776)-positive cells, were evaluated together with optic nerve damage, assessed using spectral domain-optical coherence tomography. The pharmacokinetic properties of ANX776 in both healthy and OHT model monkeys were also examined.

Results

Sustained elevation of IOP and subsequent thinning of the retinal nerve fiber layer thickness (RNFLT

SUBMITTER: Ishikawa T 

PROVIDER: S-EPMC10810027 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature