3D evaluation of the extracellular matrix of hypoxic pancreatic islets using light sheet fluorescence microscopy.

ABSTRACT: Pancreatic islet transplantation is a promising treatment for type 1 diabetes, but the survival and function of transplanted islets are hindered by the loss of extracellular matrix (ECM) during islet isolation and by low oxygenation upon implantation. This study aimed to evaluate the impact of hypoxia on ECM using a cutting-edge imaging approach based on tissue clearing and 3D microscopy. Human and rat islets were cultured under normoxic (O₂ 21%) or hypoxic (O₂ 1%) conditions. Immunofluorescence staining targeting insulin, glucagon, CA9 (a hypoxia marker), ECM proteins (collagen 4, fibronectin, laminin), and E-cadherin (intercellular adhesion protein) was performed on fixed whole islets. The cleared islets were imaged using Light Sheet Fluorescence Microscopy (LSFM) a

SUBMITTER: Ramirez M 

PROVIDER: S-EPMC10810165 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature