Endoplasmic reticulum aminopeptidase 2 regulates CD4⁺ T cells pyroptosis in rheumatoid arthritis.

ABSTRACT:

Objective

Rheumatoid arthritis (RA) is a chronic, progressive autoimmune disease with a complex pathogenesis that has not yet been fully elucidated, and T-cell pyroptosis is an important pathogenetic factor in RA. This study aimed to investigate the role of endoplasmic reticulum aminopeptidase 2 (ERAP2) in the pyroptosis of CD4⁺ T cells in RA and the specific molecular mechanism.

Methods

Peripheral venous blood was collected from human subjects, and CD4⁺ T cells were isolated and activated to measure the level of pyroptosis and ERAP2 expression. Pyroptosis levels were assessed using immunofluorescence, flow cytometry, qRT-PCR, and Western blotting. Changes in pyroptosis levels were observed upon knockdown or overexpression of ERAP2. To detect activated

SUBMITTER: Zhang J 

PROVIDER: S-EPMC10810225 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature