Elimination of 15N-thymidine after oral administration in human infants.

ABSTRACT:

Background

We have developed a new clinical research approach for the quantification of cellular proliferation in human infants to address unanswered questions about tissue renewal and regeneration. The approach consists of oral 15N-thymidine administration to label cells in S-phase, followed by Multi-isotope Imaging Mass Spectrometry for detection of the incorporated label in cell nuclei. To establish the approach, we performed an observational study to examine uptake and elimination of 15N-thymidine. We compared at-home label administration with in-hospital administration in infants with tetralogy of Fallot, a form of congenital heart disease, and infants with heart failure.

Methods

We examined urine samples from 18 infants who received 15N-thymidine (50 mg/kg body weight)

SUBMITTER: Ammanamanchi N 

PROVIDER: S-EPMC10810423 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature