Murine leukemia virus infection of non-dividing dendritic cells is dependent on nucleoporins.

ABSTRACT: Retroviral reverse transcription starts within the capsid and uncoating and reverse transcription are mutually dependent. There is still debate regarding the timing and cellular location of HIV's uncoating and reverse transcription and whether it occurs solely in the cytoplasm, nucleus or both. HIV can infect non-dividing cells because there is active transport of the preintegration complex (PIC) across the nuclear membrane, but Murine Leukemia Virus (MLV) is thought to depend on cell division for replication and whether MLV uncoating and reverse transcription is solely cytoplasmic has not been studied. Here, we used NIH3T3 and primary mouse dendritic cells to determine where the different stages of reverse transcription occur and whether cell division is needed for nuclear entry. Our data

SUBMITTER: Salas-Briceno K 

PROVIDER: S-EPMC10810426 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature