Dynamics of N6-methyladenosine modification during aging and their potential roles in the degeneration of intervertebral disc.

ABSTRACT:

Background

The N6-methyladenosine (m6A) dynamics in the progression of intervertebral disc (IVD) aging remain largely unknown. This study aimed to explore the distribution and pattern of m⁶A modification in nucleus pulpous (NP) tissues of rats at different ages.

Methods

Histological staining and MRI were performed to evaluate the degeneration of IVD. The expression of m6A modifiers was analyzed using qRT-PCR and western blot. Subsequently, methylated RNA immunoprecipitation next generation sequencing and RNA-seq were conducted to identify differences in m6A methylome and transcriptome of NP tissues.

Results

Compared to 2-month-old rats, we found significant changes in the global m6A level and the expression of Mettl3 and FTO in NP tissues from 20-month-old

SUBMITTER: Liu L 

PROVIDER: S-EPMC10810761 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature