Transcriptional characterization of iPSC-derived microglia as a model for therapeutic development in neurodegeneration.

ABSTRACT: Microglia are the resident immune cells in the brain that play a key role in driving neuroinflammation, a hallmark of neurodegenerative disorders. Inducible microglia-like cells have been developed as an in vitro platform for molecular and therapeutic hypothesis generation and testing. However, there has been no systematic assessment of similarity of these cells to primary human microglia along with their responsiveness to external cues expected of primary cells in the brain. In this study, we performed transcriptional characterization of commercially available human inducible pluripotent stem cell (iPSC)-derived microglia-like (iMGL) cells by bulk and single cell RNA sequencing to assess their similarity with primary human microglia. To evaluate their stimulation responsiveness, iMGL cell

SUBMITTER: Ramaswami G 

PROVIDER: S-EPMC10810793 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature