The m⁶A demethylase FTO targets POLQ to promote ccRCC cell proliferation and genome stability maintenance.

ABSTRACT:

Background and aim

As the first identified m⁶A demethylase, FTO has been implicated in the progression of various cancers. However, the specific mechanism of FTO in clear cell renal cell carcinoma (ccRCC) remains incompletely understood. In this study, we aimed to explore the potential molecular mechanisms influencing the progression of ccRCC.

Methods

We initially assessed the expression of FTO in tumor and adjacent tissues using TCGA database, RT-qPCR, and Western blot. We then conducted CCK-8, cell cycle analysis, and colony formation assay to investigate the impact of FTO on ccRCC cell proliferation. MeRIP-seq and RNA-seq were employed to identify potential downstream targets of FTO in ccRCC, and these findings were further validated through dual-luciferase rep

SUBMITTER: He Y 

PROVIDER: S-EPMC10810938 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature