Analysis of gene expression changes during lipid droplet formation in HepG2 human liver cancer cells.

ABSTRACT: Fatty liver is a condition of excessive triglyceride accumulation in hepatocytes. Additionally, hepatocytes exhibit a high degree of fat droplet accumulation during excessive alcohol consumption and metabolic syndrome. However, the molecular mechanisms involved in fat droplet formation remain unknown. The present study used an in vitro fatty liver formation model of the human liver cancer cell line, HepG2, to comprehensively search for fat droplet formation-related genes, and which exhibit changes in expression during fat droplet formation. Microarray analysis with extracted total RNA determined the genes that are involved in fat droplet formation and their expression was confirmed using quantitative polymerase chain reaction following the culture of the HepG2 cells in culture mediu

SUBMITTER: Chiba M 

PROVIDER: S-EPMC10811445 | biostudies-literature | 2024 Jan-Feb

REPOSITORIES: biostudies-literature