Affinity-Directed Site-Specific Protein Labeling and Its Application to Antibody-Drug Conjugates.

ABSTRACT: Chemically modified proteins have diverse applications; however, conventional chemo-selective methods often yield heterogeneously labeled products. To address this limitation, site-specific protein labeling holds significant potential, driving extensive research in this area. Nevertheless, site-specific modification of native proteins remains challenging owing to the complexity of their functional groups. Therefore, a method for site-selective labeling of intact proteins is aimed to design. In this study, a novel approach to traceless affinity-directed intact protein labeling is established, which leverages small binding proteins and genetic code expansion technology. By applying this method, a site-specific antibody labeling with a drug, which leads to the production of highly effective a

SUBMITTER: Kim S 

PROVIDER: S-EPMC10811483 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature