Characterization, modeling, and anticancer activity of L.arginase production from marine Bacillus licheniformis OF2.

ABSTRACT:

Background

L-arginase, is a powerful anticancer that hydrolyzes L-arginine to L-ornithine and urea. This enzyme is widely distributed and expressed in organisms like plants, fungi, however very scarce from bacteria. Our study is based on isolating, purifying, and screening the marine bacteria that can produce arginase.

Results

The highest arginase producing bacteria will be identified by using microbiological and molecular biology methods as Bacillus licheniformis OF2. Characterization of arginase is the objective of this study. The activity of enzyme was screened, and estimated beside partial sequencing of arginase gene was analyzed. In silico homology modeling was applied to generate the protein's 3D structure, and COACH and COFACTOR were applied to determine the protein's

SUBMITTER: Selim MS 

PROVIDER: S-EPMC10811824 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature