CircRAD23B promotes proliferation and carboplatin resistance in ovarian cancer cell lines and organoids.

ABSTRACT:

Background

Circular RNAs (circRNAs) are involved in the regulation of progression and drug resistance in ovarian cancer (OC). In the present study, we aimed to explore the role of circRAD23B, a newly identified circRNA, in the regulation of carboplatin-resistant OC.

Methods

CircRAD23B expression levels were measured using qRT-PCR. The biological roles of circRAD23B were analysed using CCK-8, colony formation, EDU, flow cytometry, and cell viability assays. RNA pull-down and luciferase assays were used to investigate the interactions of circRAD23B with mRNAs and miRNAs.

Results

CircRAD23B was significantly increased in carboplatin-resistant OC tissues. CircRAD23B promoted proliferation and reduced sensitivity to carboplatin in cell lines and patient-derived organoids (

SUBMITTER: Wang H 

PROVIDER: S-EPMC10811902 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature