Composition of lymphocyte subpopulations in normal and mildly reactive peripheral lymph nodes in cats.

ABSTRACT:

Objectives

Flow cytometric (FCM) immunophenotyping of lymphoid tissue aspirates is an available adjunct for feline lymphoma diagnostics. Reference data have only been established for feline peripheral blood. Studies investigating the composition of normal and mildly reactive feline lymph nodes (LNs) are lacking. The aim of this prospective study was to establish reference data for lymphocyte subpopulations in normal and mildly reactive feline peripheral LNs using a standardised multicolour panel of antibodies.

Methods

Macroscopically inconspicuous mandibular and/or popliteal LNs from 31 adult cats, which were euthanased for reasons other than haematological diseases, were excised and processed within 5 h after death. Multicolour flow cytometry using eight different feline-sp

SUBMITTER: Rutgen BC 

PROVIDER: S-EPMC10812179 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature