Peroxidasin Inhibition by Phloroglucinol and Other Peroxidase Inhibitors.

ABSTRACT: Human peroxidasin (PXDN) is a ubiquitous peroxidase enzyme expressed in most tissues in the body. PXDN represents an interesting therapeutic target for inhibition, as it plays a role in numerous pathologies, including cardiovascular disease, cancer and fibrosis. Like other peroxidases, PXDN generates hypohalous acids and free radical species, thereby facilitating oxidative modifications of numerous biomolecules. We have studied the inhibition of PXDN halogenation and peroxidase activity by phloroglucinol and 14 other peroxidase inhibitors. Although a number of compounds on their own potently inhibited PXDN halogenation activity, only five were effective in the presence of a peroxidase substrate with IC₅₀ values in the low μM range. Using sequential stopped-flow spectrophotometry

SUBMITTER: Paumann-Page M 

PROVIDER: S-EPMC10812467 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature