A peptide encoded by the circular form of the SHPRH gene induces apoptosis in neuroblastoma cells.

ABSTRACT:

Background

Circular RNAs (circRNAs) and their derived peptides represent largely unchartered areas in cellular biology, with many potential roles yet to be discovered. This study aimed to elucidate the role and molecular interactions of circSHPRH and its peptide derivative SHPRH-146aa in the pathogenesis of neuroblastoma (NB).

Methods

NB samples in the GSE102285 dataset were analyzed to measure circSHPRH expression, followed by in vitro experiments for validation. The role of SHPRH-146aa in NB cell proliferation, migration, and invasion was then examined, and luciferase activity assay was performed after SHPRH-146aa and RUNX1 transfection. Finally, the regulation of NB cell apoptosis by SHPRH-146aa combined with NFKBIA was tested.

Results

The GSE102285 dataset

SUBMITTER: Gao J 

PROVIDER: S-EPMC10812589 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature