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Correlation between Peptacetobacter hiranonis, the baiCD Gene, and Secondary Bile Acids in Dogs.


ABSTRACT: Bile acid metabolism is a key pathway modulated by intestinal microbiota. Peptacetobacter (Clostridium) hiranonis has been described as the main species responsible for the conversion of primary into secondary fecal unconjugated bile acids (fUBA) in dogs. This multi-step biochemical pathway is encoded by the bile acid-inducible (bai) operon. We aimed to assess the correlation between P. hiranonis abundance, the abundance of one specific gene of the bai operon (baiCD), and secondary fUBA concentrations. In this retrospective study, 133 fecal samples were analyzed from 24 dogs. The abundances of P. hiranonis and baiCD were determined using qPCR. The concentration of fUBA was measured by gas chromatography-mass spectrometry. The baiCD abundance exhibited a strong positive correlation with secondary fUBA (ρ = 0.7377, 95% CI (0.6461, 0.8084), p < 0.0001). Similarly, there was a strong correlation between P. hiranonis and secondary fUBA (ρ = 0.6658, 95% CI (0.5555, 0.7532), p < 0.0001). Animals displaying conversion of fUBA and lacking P. hiranonis were not observed. These results suggest P. hiranonis is the main converter of primary to secondary bile acids in dogs.

SUBMITTER: Correa Lopes B 

PROVIDER: S-EPMC10812727 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature

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Correlation between <i>Peptacetobacter hiranonis</i>, the <i>baiCD</i> Gene, and Secondary Bile Acids in Dogs.

Correa Lopes Bruna B   Chen Chih-Chun CC   Sung Chi-Hsuan CH   Ishii Patricia Eri PE   Medina Luis Fernando da Costa LFDC   Gaschen Frederic P FP   Suchodolski Jan S JS   Pilla Rachel R  

Animals : an open access journal from MDPI 20240109 2


Bile acid metabolism is a key pathway modulated by intestinal microbiota. <i>Peptacetobacter</i> (<i>Clostridium</i>) <i>hiranonis</i> has been described as the main species responsible for the conversion of primary into secondary fecal unconjugated bile acids (fUBA) in dogs. This multi-step biochemical pathway is encoded by the bile acid-inducible (<i>bai</i>) operon. We aimed to assess the correlation between <i>P. hiranonis</i> abundance, the abundance of one specific gene of the <i>bai</i> o  ...[more]

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