Neurofilament Light Protein Rod Domain Exhibits Structural Heterogeneity.

ABSTRACT: Neurofilaments are neuron-specific proteins that belong to the intermediate filament (IFs) protein family, with the neurofilament light chain protein (NFL) being the most abundant. The IFs structure typically includes a central coiled-coil rod domain comprised of coils 1A, 1B, and 2, separated by linker regions. The thermal stability of the IF molecule plays a crucial role in its ability for self-association. In the current study, we investigated the thermal stability of NFL coiled-coil domains by analyzing a set of recombinant domains and their fusions (NFL_1B, NFL_1A+1B, NFL₂, NFL_1B+2, and NFL_ROD) via circular dichroism spectroscopy and differential scanning calorimetry. The thermal stability of coiled-coil domains is evident in a wide

SUBMITTER: Nefedova VV 

PROVIDER: S-EPMC10813002 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature