Perfusable Tissue Bioprinted into a 3D-Printed Tailored Bioreactor System.

ABSTRACT: Bioprinting provides a powerful tool for regenerative medicine, as it allows tissue construction with a patient's specific geometry. However, tissue culture and maturation, commonly supported by dynamic bioreactors, are needed. We designed a workflow that creates an implant-specific bioreactor system, which is easily producible and customizable and supports cell cultivation and tissue maturation. First, a bioreactor was designed and different tissue geometries were simulated regarding shear stress and nutrient distribution to match cell culture requirements. These tissues were then directly bioprinted into the 3D-printed bioreactor. To prove the ability of cell maintenance, C2C12 cells in two bioinks were printed into the system and successfully cultured for two weeks. Next, human mesenchy

SUBMITTER: Gensler M 

PROVIDER: S-EPMC10813239 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature