SOX2 and SOX9 Expression in Developing Postnatal Opossum (Monodelphis domestica) Cortex.

ABSTRACT: (1) Background: Central nervous system (CNS) development is characterized by dynamic changes in cell proliferation and differentiation. Key regulators of these transitions are the transcription factors such as SOX2 and SOX9. SOX2 is involved in the maintenance of progenitor cell state and neural stem cell multipotency, while SOX9, expressed in neurogenic niches, plays an important role in neuron/glia switch with predominant expression in astrocytes in the adult brain. (2) Methods: To validate SOX2 and SOX9 expression patterns in developing opossum (Monodelphis domestica) cortex, we used immunohistochemistry (IHC) and the isotropic fractionator method on fixed cortical tissue from comparable postnatal ages, as well as dissociated primary neuronal cultures. (3) Results: Neurons positi

SUBMITTER: Baricevic Z 

PROVIDER: S-EPMC10813269 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature